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LearnDM1208
antibody from Acris Antibodies GmbH
Targeting: GZMB
CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT
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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [3]
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- Product number
- DM1208 - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#DM1208, RRID:AB_1002005
- Product name
- anti Granzyme B (GZMB)
- Antibody type
- Monoclonal
- Antigen
- Genetic immunisation with cDNA encoding human Granzyme B
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- GM4C1
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure.1: FACS analysis of BOSC23 cells using Granzyme B antibody (Cat#DM1208). BOSC23 cells were transiently trans-fected with an expression vector encoding either Granzyme B (red curve) or an irrelevant protein (controlt-ransfectant: black curve). Binding of GM-4C1 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with Granzyme B trans-fected cells.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure.2: Intracellular detection of Granzyme B in human PBMC by FACS analysis using Granzyme B antibody. PBMC were cultivated in the presence of phorbol ester and ionomycin subsequently fixed and permeabilised. Binding of Granzyme B antibody was detected with a FITC-conjugated secondary antibody.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure.3: BOSC cells were transiently transfected with expression vectors for Granzyme A, B, K, or M. Expression of the constructs was tested with an anti-tag monoclonal antibody (green curves), an irrelevant monoclonal antibody served as negative control (black curves). For specificity testing, purified Granzyme B antibody was tested on all transfectants. A positive signal wasobtained only with Granzyme B transfected cells (red curves).
