IC2906G

antibody from R&D Systems

Targeting: GZMB CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC2906G

Provider

R&D Systems

Product name

Human Granzyme B Alexa Fluor® 488-conjugated Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Granzyme B in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) Granzyme A, rhGranzyme H, recombinant mouse (rm) Granzyme B, rmGranzyme C, rmGranzyme D, or rmGranzyme G.

Reactivity

Human

Host

Mouse

Conjugate

Green dye

Antigen sequence

P10144


Isotype

IgG

Antibody clone number

351927

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

References

Submitted references

The IL-7Rα pathway is quantitatively and functionally altered in CD8 T cells in multiple sclerosis.

Kreft KL, Verbraak E, Wierenga-Wolf AF, van Meurs M, Oostra BA, Laman JD, Hintzen RQ
Journal of immunology (Baltimore, Md. : 1950) 2012 Feb 15;188(4):1874-83

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Granzyme B in NK-92 Human Cell Line by Flow Cytometry. NK-92 human natural killer lymphoma cell line was stained with Mouse Anti-Human Granzyme B Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC2906G, filled histogram) or isotype control antibody (Catalog # IC003G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.