PA5-97022

antibody from Invitrogen Antibodies

Targeting: MDC1 Em:AB023051.5, KIAA0170, NFBD1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-97022

Provider

Invitrogen Antibodies

Product name

MDC1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MEDTQAIDWD VEEEEETEQS SESLRCNVEP VGRLHIFSGA HGPEKDFPLH LGKNVVGRMP DCSVALPFPS ISKQHAEIEI LAWDKAPILR DCGSLNGTQI LRPPKVLSPG VSHRLRDQEL ILFADLLCQY HRLDVSLPFV SRGPLTVEET PRVQGETQPQ RLLLAEDSEE EVDFLSERRM VKKSRTTSSS VIVPESDEEG HSPVLGGLGP PFAFNLNSDT DVEEGQQPAT EEASSAARRG ATVEAKQSEA EVVTEIQLEK DQPLVKERDN DTKVKRGAGN GVVPAGVILE RSQPPGEDSD TDVDDDSRPP GRPAEVHLER AQPFGFIDSD TDAEEERIPA TPVVIPMKKR; Positive Samples: Jurkat, LO2; Cellular Location: Chromosome, Nucleus

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.28 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

O-GlcNAcylation Affects the Pathway Choice of DNA Double-Strand Break Repair.

Averbek S, Jakob B, Durante M, Averbeck NB
International journal of molecular sciences 2021 May 27;22(11)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of MDC1 in extracts of various cell lines using MDC1 Polyclonal Antibody (Product # PA5-97022) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of MDC1 was performed in U-2 OS cells using MDC1 Polyclonal Antibody (Product # PA5-97022) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Mediator of DNA damage checkpoint protein 1 was performed using 70% confluent log phase HCT 116 Nocodazole treated (200 nmol/L for 16hrs). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MDC1 Polyclonal Antibody (Product # PA5-97022) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight, and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Nuclear foci localization. Panel e represents untreated cells with nuclear localization. Panel f represents control cells with no primary antibody to assess the background. The images were captured at 60X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of MDC1 in paraffin-embedded human spleen. Samples were incubated with MDC1 Polyclonal antibody (Product # PA5-97022) using a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of MDC1 in paraffin-embedded human testis. Samples were incubated with MDC1 Polyclonal antibody (Product # PA5-97022) using a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.