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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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- Product number
- PA1-41131 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MDC1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Suggested positive control: Jurkat whole cell lysate.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NFBD1 in 30 ugs of Jurkat cell lysate using a NFBD1 polyclonal antibody (Product # PA1-41131) at 1:1000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MDC1 in 30 µg Jurkat cell lysate. Samples were incubated in MDC1 polyclonal antibody (Product # PA1-41131) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-MDC1 Polyclonal Antibody (Product # PA1-41131) and a 250kDakDa band corresponding to Mediator of DNA damage checkpoint protein 1 was observed across the cell lines tested. Nuclear enriched extracts (30 µg lysate) of HeLa (Lane 1), A-431 (Lane 2), HCT 116 (Lane 3), Mouse Testis (Lane 4) and Rat Testis (Lane 5) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Reagent Kit (Product # WP20005).
