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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NB100-397 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-397, RRID:AB_2143264
- Product name
- Rabbit Polyclonal MDC1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references JMJD6 modulates DNA damage response through downregulating H4K16ac independently of its enzymatic activity.
RNF8- and RNF168-dependent degradation of KDM4A/JMJD2A triggers 53BP1 recruitment to DNA damage sites.
Huo D, Chen H, Cheng Y, Song X, Zhang K, Li MJ, Xuan C
Cell death and differentiation 2020 Mar;27(3):1052-1066
Cell death and differentiation 2020 Mar;27(3):1052-1066
RNF8- and RNF168-dependent degradation of KDM4A/JMJD2A triggers 53BP1 recruitment to DNA damage sites.
Mallette FA, Mattiroli F, Cui G, Young LC, Hendzel MJ, Mer G, Sixma TK, Richard S
The EMBO journal 2012 Apr 18;31(8):1865-78
The EMBO journal 2012 Apr 18;31(8):1865-78
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MDC1 Antibody [NB100-397] - Detection of Human MDC1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MDC1 antibody NB100-397 (Lot 2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. MDC1 was also immunoprecipitated by Lot 1 of rabbit anti-MDC1 antibody NB100-397. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MDC1 Antibody [NB100-397] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MDC1
