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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Flow cytometry [1]
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- Product number
- FAB190F-025 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CXCR5 Fluorescein-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Stains human CXCR5 transfectants but not the parental cell lines in flow cytometry. Does not cross-react with human CXCR2, CXCR3, or CXCR4 transfectants.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
P32302- Isotype
- IgG
- Antibody clone number
- 51505
- Vial size
- 25 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Glatiramer Acetate, Dimethyl Fumarate, and Monomethyl Fumarate Upregulate the Expression of CCR10 on the Surface of Natural Killer Cells and Enhance Their Chemotaxis and Cytotoxicity.
CD4+ T cell-derived IL-21 and deprivation of CD40 signaling favor the in vivo development of granzyme B-expressing regulatory B cells in HIV patients.
CXCR5⁺ T helper cells mediate protective immunity against tuberculosis.
CCR2 identifies a stable population of human effector memory CD4+ T cells equipped for rapid recall response.
Down-modulation of CXCR3 surface expression and function in CD8+ T cells from cutaneous T cell lymphoma patients.
Maghazachi AA, Sand KL, Al-Jaderi Z
Frontiers in immunology 2016;7:437
Frontiers in immunology 2016;7:437
CD4+ T cell-derived IL-21 and deprivation of CD40 signaling favor the in vivo development of granzyme B-expressing regulatory B cells in HIV patients.
Kaltenmeier C, Gawanbacht A, Beyer T, Lindner S, Trzaska T, van der Merwe JA, Härter G, Grüner B, Fabricius D, Lotfi R, Schwarz K, Schütz C, Hönig M, Schulz A, Kern P, Bommer M, Schrezenmeier H, Kirchhoff F, Jahrsdörfer B
Journal of immunology (Baltimore, Md. : 1950) 2015 Apr 15;194(8):3768-77
Journal of immunology (Baltimore, Md. : 1950) 2015 Apr 15;194(8):3768-77
CXCR5⁺ T helper cells mediate protective immunity against tuberculosis.
Slight SR, Rangel-Moreno J, Gopal R, Lin Y, Fallert Junecko BA, Mehra S, Selman M, Becerril-Villanueva E, Baquera-Heredia J, Pavon L, Kaushal D, Reinhart TA, Randall TD, Khader SA
The Journal of clinical investigation 2013 Feb;123(2):712-26
The Journal of clinical investigation 2013 Feb;123(2):712-26
CCR2 identifies a stable population of human effector memory CD4+ T cells equipped for rapid recall response.
Zhang HH, Song K, Rabin RL, Hill BJ, Perfetto SP, Roederer M, Douek DC, Siegel RM, Farber JM
Journal of immunology (Baltimore, Md. : 1950) 2010 Dec 1;185(11):6646-63
Journal of immunology (Baltimore, Md. : 1950) 2010 Dec 1;185(11):6646-63
Down-modulation of CXCR3 surface expression and function in CD8+ T cells from cutaneous T cell lymphoma patients.
Winter D, Moser J, Kriehuber E, Wiesner C, Knobler R, Trautinger F, Bombosi P, Stingl G, Petzelbauer P, Rot A, Maurer D
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 15;179(6):4272-82
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 15;179(6):4272-82
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of CXCR5 in Human Peripheral Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human CXCR5 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB190F) or (B) Mouse IgG2B Fluorescein Isotype Control (Catalog # IC0041F). View our protocol for Staining Membrane-associated Proteins.
