Antibody data
- Antibody Data
- Antigen structure
- References [18]
- Comments [0]
- Validations
- Flow cytometry [2]
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- Product number
- FAB190P-025 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CXCR5 PE-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Stains human CXCR5 transfectants but not the parental cell lines in flow cytometry. Does not cross-react with human CXCR2, CXCR3, or CXCR4 transfectants.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
P32302
- Isotype
- IgG
- Antibody clone number
- 51505
- Vial size
- 25 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Mesenchymal stromal cell secretome up-regulates 47 kDa CXCR4 expression, and induce invasiveness in neuroblastoma cell lines.
Herpes simplex virus enhances chemokine function through modulation of receptor trafficking and oligomerization.
IL-12 directs further maturation of ex vivo differentiated NK cells with improved therapeutic potential.
IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation.
Thymoquinone inhibits the CXCL12-induced chemotaxis of multiple myeloma cells and increases their susceptibility to Fas-mediated apoptosis.
Primary B-cell deficiencies reveal a link between human IL-17-producing CD4 T-cell homeostasis and B-cell differentiation.
Increased numbers of immature plasma cells in peripheral blood specifically overexpress chemokine receptor CXCR3 and CXCR4 in patients with ulcerative colitis.
Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
Chemokine CXCL13 is overexpressed in the tumour tissue and in the peripheral blood of breast cancer patients.
BAFF enhances chemotaxis of primary human B cells: a particular synergy between BAFF and CXCL13 on memory B cells.
CD43, but not P-selectin glycoprotein ligand-1, functions as an E-selectin counter-receptor in human pre-B-cell leukemia NALL-1.
Altered expression of the receptor-ligand pair CXCR5/CXCL13 in B cells during chronic HIV-1 infection.
Evidence for HIV-associated B cell exhaustion in a dysfunctional memory B cell compartment in HIV-infected viremic individuals.
Chemokine production and chemokine receptor expression by human glioma cells: role of CXCL10 in tumour cell proliferation.
IL-21 and BAFF/BLyS synergize in stimulating plasma cell differentiation from a unique population of human splenic memory B cells.
Chemokine receptors in head and neck cancer: association with metastatic spread and regulation during chemotherapy.
Chemokine receptor expression in EBV-associated lymphoproliferation in hu/SCID mice: implications for CXCL12/CXCR4 axis in lymphoma generation.
Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
Shankar V, Hori H, Kihira K, Lei Q, Toyoda H, Iwamoto S, Komada Y
PloS one 2015;10(3):e0120069
PloS one 2015;10(3):e0120069
Herpes simplex virus enhances chemokine function through modulation of receptor trafficking and oligomerization.
Martinez-Martin N, Viejo-Borbolla A, Martín R, Blanco S, Benovic JL, Thelen M, Alcamí A
Nature communications 2015 Jan 27;6:6163
Nature communications 2015 Jan 27;6:6163
IL-12 directs further maturation of ex vivo differentiated NK cells with improved therapeutic potential.
Lehmann D, Spanholtz J, Sturtzel C, Tordoir M, Schlechta B, Groenewegen D, Hofer E
PloS one 2014;9(1):e87131
PloS one 2014;9(1):e87131
IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation.
Morandi F, Di Carlo E, Ferrone S, Petretto A, Pistoia V, Airoldi I
Journal of immunology (Baltimore, Md. : 1950) 2014 Mar 15;192(6):2634-42
Journal of immunology (Baltimore, Md. : 1950) 2014 Mar 15;192(6):2634-42
Thymoquinone inhibits the CXCL12-induced chemotaxis of multiple myeloma cells and increases their susceptibility to Fas-mediated apoptosis.
Badr G, Lefevre EA, Mohany M
PloS one 2011;6(9):e23741
PloS one 2011;6(9):e23741
Primary B-cell deficiencies reveal a link between human IL-17-producing CD4 T-cell homeostasis and B-cell differentiation.
Barbosa RR, Silva SP, Silva SL, Melo AC, Pedro E, Barbosa MP, Pereira-Santos MC, Victorino RM, Sousa AE
PloS one 2011;6(8):e22848
PloS one 2011;6(8):e22848
Increased numbers of immature plasma cells in peripheral blood specifically overexpress chemokine receptor CXCR3 and CXCR4 in patients with ulcerative colitis.
Hosomi S, Oshitani N, Kamata N, Sogawa M, Okazaki H, Tanigawa T, Yamagami H, Watanabe K, Tominaga K, Watanabe T, Fujiwara Y, Maeda K, Hirakawa K, Arakawa T
Clinical and experimental immunology 2011 Feb;163(2):215-24
Clinical and experimental immunology 2011 Feb;163(2):215-24
Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH
Journal of immunology (Baltimore, Md. : 1950) 2010 Mar 15;184(6):2886-98
Journal of immunology (Baltimore, Md. : 1950) 2010 Mar 15;184(6):2886-98
Chemokine CXCL13 is overexpressed in the tumour tissue and in the peripheral blood of breast cancer patients.
Panse J, Friedrichs K, Marx A, Hildebrandt Y, Luetkens T, Barrels K, Horn C, Stahl T, Cao Y, Milde-Langosch K, Niendorf A, Kröger N, Wenzel S, Leuwer R, Bokemeyer C, Hegewisch-Becker S, Atanackovic D
British journal of cancer 2008 Sep 16;99(6):930-8
British journal of cancer 2008 Sep 16;99(6):930-8
BAFF enhances chemotaxis of primary human B cells: a particular synergy between BAFF and CXCL13 on memory B cells.
Badr G, Borhis G, Lefevre EA, Chaoul N, Deshayes F, Dessirier V, Lapree G, Tsapis A, Richard Y
Blood 2008 Mar 1;111(5):2744-54
Blood 2008 Mar 1;111(5):2744-54
CD43, but not P-selectin glycoprotein ligand-1, functions as an E-selectin counter-receptor in human pre-B-cell leukemia NALL-1.
Nonomura C, Kikuchi J, Kiyokawa N, Ozaki H, Mitsunaga K, Ando H, Kanamori A, Kannagi R, Fujimoto J, Muroi K, Furukawa Y, Nakamura M
Cancer research 2008 Feb 1;68(3):790-9
Cancer research 2008 Feb 1;68(3):790-9
Altered expression of the receptor-ligand pair CXCR5/CXCL13 in B cells during chronic HIV-1 infection.
Cagigi A, Mowafi F, Phuong Dang LV, Tenner-Racz K, Atlas A, Grutzmeier S, Racz P, Chiodi F, Nilsson A
Blood 2008 Dec 1;112(12):4401-10
Blood 2008 Dec 1;112(12):4401-10
Evidence for HIV-associated B cell exhaustion in a dysfunctional memory B cell compartment in HIV-infected viremic individuals.
Moir S, Ho J, Malaspina A, Wang W, DiPoto AC, O'Shea MA, Roby G, Kottilil S, Arthos J, Proschan MA, Chun TW, Fauci AS
The Journal of experimental medicine 2008 Aug 4;205(8):1797-805
The Journal of experimental medicine 2008 Aug 4;205(8):1797-805
Chemokine production and chemokine receptor expression by human glioma cells: role of CXCL10 in tumour cell proliferation.
Maru SV, Holloway KA, Flynn G, Lancashire CL, Loughlin AJ, Male DK, Romero IA
Journal of neuroimmunology 2008 Aug 13;199(1-2):35-45
Journal of neuroimmunology 2008 Aug 13;199(1-2):35-45
IL-21 and BAFF/BLyS synergize in stimulating plasma cell differentiation from a unique population of human splenic memory B cells.
Ettinger R, Sims GP, Robbins R, Withers D, Fischer RT, Grammer AC, Kuchen S, Lipsky PE
Journal of immunology (Baltimore, Md. : 1950) 2007 Mar 1;178(5):2872-82
Journal of immunology (Baltimore, Md. : 1950) 2007 Mar 1;178(5):2872-82
Chemokine receptors in head and neck cancer: association with metastatic spread and regulation during chemotherapy.
Muller A, Sonkoly E, Eulert C, Gerber PA, Kubitza R, Schirlau K, Franken-Kunkel P, Poremba C, Snyderman C, Klotz LO, Ruzicka T, Bier H, Zlotnik A, Whiteside TL, Homey B, Hoffmann TK
International journal of cancer 2006 May 1;118(9):2147-57
International journal of cancer 2006 May 1;118(9):2147-57
Chemokine receptor expression in EBV-associated lymphoproliferation in hu/SCID mice: implications for CXCL12/CXCR4 axis in lymphoma generation.
Piovan E, Tosello V, Indraccolo S, Cabrelle A, Baesso I, Trentin L, Zamarchi R, Tamamura H, Fujii N, Semenzato G, Chieco-Bianchi L, Amadori A
Blood 2005 Feb 1;105(3):931-9
Blood 2005 Feb 1;105(3):931-9
Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A
Journal of leukocyte biology 2003 May;73(5):584-90
Journal of leukocyte biology 2003 May;73(5):584-90
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CXCR5 in CD19+ Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4867F) and either (A) Mouse Anti-Human CXCR5 PE-conjugated Monoclonal Antibody (Catalog # FAB190P) or (B) Mouse IgG2B control antibody (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CXCR5 in CD4+ Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A) and either (A) Mouse Anti-Human CXCR5 PE-conjugated Monoclonal Antibody (Catalog # FAB190P) or (B) Mouse IgG2B isotype control antibody (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.