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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Flow cytometry [2]
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Validation data
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- Product number
- MAB6198 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CXCR5 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects recombinant mouse CXCR5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse CXCR7 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q04683- Isotype
- IgG
- Antibody clone number
- 614641
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CXCR5 in A20 Mouse Cell Line. CXCR5 was detected in immersion fixed A20 mouse B cell lymphoma cell line using Rat Anti-Mouse CXCR5 Monoclonal Antibody (Catalog # MAB6198) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Rat IgG Secondary Antibody (green; Catalog # NL015) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CXCR5 in T Follicular Helper Cells by Flow Cytometry. T follicular helper cells from immunized Balb/c mice were stained with Rat Anti-Mouse CD4 Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # FAB554V) and either (A) Rat Anti-Mouse CXCR5 Monoclonal Antibody (Catalog # MAB6198) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CXCR5 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse CXCR5 Monoclonal Antibody (Catalog # MAB6198) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).
