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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- AF6198 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CXCR5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse CXCR5 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse CXCR7 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q04683- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of CXCR5 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Mouse CXCR5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6198) followed by Fluorescein-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0109) and Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A). Quadrant markers were set based on control antibody staining (Catalog # AB-108-C).
