Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 14-9185-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD185 (CXCR5) Monoclonal Antibody (MU5UBEE), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The MU5UBEE monoclonal antibody reacts with Human CD185. CD185, which is also known as C-X-C chemokine receptor 5 (CXCR5) and Burkitt lymphoma receptor 1 (BLR1), is a seven transmembrane G protein-coupled receptor originally identified in Burkitt's lymphoma. In peripheral blood, CXCR5 is expressed on B cells, CD4+ T cells (but not Th1 or Th2 cells), as well as on a subpopulation of memory (CD45RO+) T cells. CXCR5+ circulating T cells are in a resting state and migrate to the lymph nodes due to expression of CCR7 and CD62L. In tonsil, CXCR5 is expressed on nearly all CD4+ cells along with CD45RO and such activation markers as CD69 and ICOS. Tonsillar CXCR5+ cells have been shown to induce antibody production when co-cultured with B cells, thus supporting their role in providing B cell help. Furthermore, this chemokine receptor plays a critical role in lymphocyte trafficking, in particular T cell migration into the B cell follicles of germinal centers in response to CXCL13, making CXCR5 an established marker of follicular helper T cells. Applications Reported: This MU5UBEE antibody has been reported for use in flow cytometric analysis. Applications Tested: This MU5UBEE antibody has been tested by flow cytometric analysis of human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- MU5UBEE
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Elicitation of Robust Tier 2 Neutralizing Antibody Responses in Nonhuman Primates by HIV Envelope Trimer Immunization Using Optimized Approaches.
Perturbed CD8+ T cell TIGIT/CD226/PVR axis despite early initiation of antiretroviral treatment in HIV infected individuals.
Dynamics of SIV-specific CXCR5+ CD8 T cells during chronic SIV infection.
Follicular Regulatory CD8 T Cells Impair the Germinal Center Response in SIV and Ex Vivo HIV Infection.
Cytokine-secreting follicular T cells shape the antibody repertoire.
Systemic immunoregulatory and pathogenic functions of homeostatic chemokine receptors.
A putative chemokine receptor, BLR1, directs B cell migration to defined lymphoid organs and specific anatomic compartments of the spleen.
Sequence variation of a novel heptahelical leucocyte receptor through alternative transcript formation.
Differentiation-specific expression of a novel G protein-coupled receptor from Burkitt's lymphoma.
Pauthner M, Havenar-Daughton C, Sok D, Nkolola JP, Bastidas R, Boopathy AV, Carnathan DG, Chandrashekar A, Cirelli KM, Cottrell CA, Eroshkin AM, Guenaga J, Kaushik K, Kulp DW, Liu J, McCoy LE, Oom AL, Ozorowski G, Post KW, Sharma SK, Steichen JM, de Taeye SW, Tokatlian T, Torrents de la Peña A, Butera ST, LaBranche CC, Montefiori DC, Silvestri G, Wilson IA, Irvine DJ, Sanders RW, Schief WR, Ward AB, Wyatt RT, Barouch DH, Crotty S, Burton DR
Immunity 2017 Jun 20;46(6):1073-1088.e6
Immunity 2017 Jun 20;46(6):1073-1088.e6
Perturbed CD8+ T cell TIGIT/CD226/PVR axis despite early initiation of antiretroviral treatment in HIV infected individuals.
Tauriainen J, Scharf L, Frederiksen J, Naji A, Ljunggren HG, Sönnerborg A, Lund O, Reyes-Terán G, Hecht FM, Deeks SG, Betts MR, Buggert M, Karlsson AC
Scientific reports 2017 Jan 13;7:40354
Scientific reports 2017 Jan 13;7:40354
Dynamics of SIV-specific CXCR5+ CD8 T cells during chronic SIV infection.
Mylvaganam GH, Rios D, Abdelaal HM, Iyer S, Tharp G, Mavigner M, Hicks S, Chahroudi A, Ahmed R, Bosinger SE, Williams IR, Skinner PJ, Velu V, Amara RR
Proceedings of the National Academy of Sciences of the United States of America 2017 Feb 21;114(8):1976-1981
Proceedings of the National Academy of Sciences of the United States of America 2017 Feb 21;114(8):1976-1981
Follicular Regulatory CD8 T Cells Impair the Germinal Center Response in SIV and Ex Vivo HIV Infection.
Miles B, Miller SM, Folkvord JM, Levy DN, Rakasz EG, Skinner PJ, Connick E
PLoS pathogens 2016 Oct;12(10):e1005924
PLoS pathogens 2016 Oct;12(10):e1005924
Cytokine-secreting follicular T cells shape the antibody repertoire.
Reinhardt RL, Liang HE, Locksley RM
Nature immunology 2009 Apr;10(4):385-93
Nature immunology 2009 Apr;10(4):385-93
Systemic immunoregulatory and pathogenic functions of homeostatic chemokine receptors.
Müller G, Höpken UE, Stein H, Lipp M
Journal of leukocyte biology 2002 Jul;72(1):1-8
Journal of leukocyte biology 2002 Jul;72(1):1-8
A putative chemokine receptor, BLR1, directs B cell migration to defined lymphoid organs and specific anatomic compartments of the spleen.
Förster R, Mattis AE, Kremmer E, Wolf E, Brem G, Lipp M
Cell 1996 Dec 13;87(6):1037-47
Cell 1996 Dec 13;87(6):1037-47
Sequence variation of a novel heptahelical leucocyte receptor through alternative transcript formation.
Barella L, Loetscher M, Tobler A, Baggiolini M, Moser B
The Biochemical journal 1995 Aug 1;309 ( Pt 3):773-9
The Biochemical journal 1995 Aug 1;309 ( Pt 3):773-9
Differentiation-specific expression of a novel G protein-coupled receptor from Burkitt's lymphoma.
Dobner T, Wolf I, Emrich T, Lipp M
European journal of immunology 1992 Nov;22(11):2795-9
European journal of immunology 1992 Nov;22(11):2795-9
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0199-42) and 0.125 µg of Mouse IgG2b K Isotype Control Purified (Product # 14-4732-82) (left) or 0.125 µg of Anti-Human CD185 (CXCR5) Purified (right) followed by F (ab')2 Anti-Mouse IgG PE (Product # 12-4010-82).Cells in the lymphocyte gate were used for analysis.