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Western blot
Immunoelectron microscopyAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- PA5-111766 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CXCR5 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Description: The 61D3 monoclonal antibody reacts with human CD14, a 53-55 kDa GPI-linked glycoprotein. CD14 is expressed on monocytes, interfollicular macrophages and some dendritic cells. Complexes of LPS and LBP (LPS-Binding Protein) bind with high affinity to monocytes through the surface CD14.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.85 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CXCR5 was performed in Burkitts lymphoma (Raji) (lanes 1 and 4), human prostate carcinoma (LNCaP) (lanes 2 and 5) and human prostate carcinoma (PC-3) cell line lysates. Lane 1-3: CXCR5 (extracellular) Antibody (Product # PA5-111766) at a dilution of 1:400. Lane 4-6: CXCR5 (extracellular) Antibody preincubated with the negative control antigen.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CXCR5 was performed in Burkitts lymphoma (Raji) (lanes 1 and 4), human prostate carcinoma (LNCaP) (lanes 2 and 5) and human prostate carcinoma (PC-3) cell line lysates. Lane 1-3: CXCR5 (extracellular) Antibody (Product # PA5-111766) at a dilution of 1:400. Lane 4-6: CXCR5 (extracellular) Antibody preincubated with the negative control antigen.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of CXCR5 in human LNCaP cells. A) Extracellular staining of cells using CXCR5 (extracellular) Antibody (Product # PA5-111766) (green), (1:25) merged with live view of the cells (magnitude X40). B) Extracellular staining of LNCaP cells merged with live view of the cells (magnitude X100).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of CXCR5 in human promyelocytic leukemia (HL-60) live intact cells. Black: Unstained cells. Green: Cells and CXCR5 (extracellular) Antibody (Product # PA5-111766), (5 µg/0.5x10^6 cells).
