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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [2]
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- Product number
- H037T02Y03 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD185 (CXCR5) Monoclonal Antibody (MU5UBEE), NovaFluor™ Yellow 610, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The MU5UBEE monoclonal antibody reacts with human and non-human primate CD185. CD185, which is also known as C-X-C chemokine receptor 5 (CXCR5) and Burkitt lymphoma receptor 1 (BLR1), is a seven transmembrane G protein-coupled receptor originally identified in Burkitt's lymphoma. In peripheral blood, CD185 is expressed on B cells, CD4+ T cells (but not Th1 or Th2 cells), as well as on a subpopulation of memory (CD45RO+) T cells. Circulating CD185+ T cells are in a resting state and migrate to the lymph nodes due to expression of CCR7 and CD62L. In tonsil, CD185 is expressed on nearly all CD4+ cells together with CD45RO and activation markers such as CD69 and ICOS. Tonsillar CD185+ cells have been shown to induce antibody production when co-cultured with B cells, thus supporting their role in providing help to B cells. Furthermore, this chemokine receptor plays a critical role in lymphocyte trafficking, in particular T cell migration into the B cell follicles of germinal centers in response to CXCL13, making CD185 an established marker of follicular helper T cells. The MU5UBEE monoclonal antibody also crossreacts with Rhesus macaque.
- Antibody clone number
- MU5UBEE
- Concentration
- 0.6 µg/Test
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Normal human peripheral blood cells were unstained (left) or stained with CD185 Monoclonal Antibody, NovaFluor Yellow 610 (Product # h037t03y03) (right). All cells were co-stained with CD19 Monoclonal Antibody, eFluor 450 (Product # 48-0199-42). Total viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD Blue (Product # L34962). Data was acquired on a 5-laser Cytek Aurora and unmixed with autofluorescence extraction.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Spectral signature for NovaFluor Yellow 610 collected on a 5-laser Cytek Aurora Full Spectrum flow cytometer using Cytek assay settings. Human peripheral blood mononuclear cells were stained with anti-human CD4 (SK3) and signatures displayed following gating on the lymphocyte population.
