PA5-27170

antibody from Invitrogen Antibodies

Targeting: CXCR5 BLR1, CD185, MDR15

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27170

Provider

Invitrogen Antibodies

Product name

CXCR5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: HepG2.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using CXCR5 Polyclonal Antibody (Product # PA5-27170). Sample (30 µg of whole cell lysate). Lane A: Hep G2. 10% SDS PAGE. CXCR5 Polyclonal Antibody (Product # PA5-27170) diluted at 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CXCR5 Polyclonal Antibody detects CXCR5 protein at cell membrane by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CXCR5 stained by CXCR5 Polyclonal Antibody (Product # PA5-27170) diluted at 1:500. Blue: Fluoroshield with DAPI .

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CXCR5 Polyclonal Antibody detects CXCR5 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse skin. CXCR5 stained by CXCR5 Polyclonal Antibody (Product # PA5-27170) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CXCR5 Polyclonal Antibody detects CXCR5 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human kidney. CXCR5 stained by CXCR5 Polyclonal Antibody (Product # PA5-27170) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CXCR5 Polyclonal Antibody detects CXCR5 protein at membrane on PC13 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded PC13 xenograft. CXCR5 Polyclonal Antibody (Product # PA5-27170) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.