GTX30511

antibody from GeneTex

Targeting: KIF11 Eg5, HKSP, KNSL1, TRIP5

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX30511

Provider

GeneTex

Proper citation

GeneTex Cat#GTX30511, RRID:AB_385700

Product name

Eg5 antibody

Antibody type

Polyclonal

Host

Rabbit

Vial size

100 µl

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Anti-GST polyclonal antibody(GTX30511) in western blot shows detection of recombinant GST (indicated by band at ~ 28 kDa). The SDS-PAGE contained approximately 0.2 µg of rGST loaded on to a 4-20% gradient gel for separation. After electrophoresis, the gel was transferred to nitrocellulose and blocked with “Blocking Buffer for Fluorescent Western Blotting” in TBS for 1h at RT. The membrane was probed with anti-GST antibody at a 1:2,000 dilution in blocking reagent, overnight at 4° C. For detection DyLight™800 conjugated Donkey-a-Goat IgG2 was used at a 1:20,000 dilution (in blocking reagent) for 30 min at 25° C.

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunoprecipitation of anti-GST antibody (GTX30511). Lane 1: T98G cells incubated with GST-Pin1. Lane 2: T98G cells incubated with GST-CDK4/cyclinD1. Lane 3: T98G cells incubated with GST-Pin1 and GST-CDK4/cyclinD1. Immunoprecipitated with pRb antibody. Load: 25 µg per lane. Primary antibody: anti-GST 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.

Validation comment

WB