Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- GTX30692 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX30692, RRID:AB_625879
- Product name
- Eg5 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Drosophila, Porcine, Simian
- Host
- Rabbit
Submitted references Nek9 Phosphorylation Defines a New Role for TPX2 in Eg5-Dependent Centrosome Separation before Nuclear Envelope Breakdown.
Eibes S, Gallisà-Suñé N, Rosas-Salvans M, Martínez-Delgado P, Vernos I, Roig J
Current biology : CB 2018 Jan 8;28(1):121-129.e4
Current biology : CB 2018 Jan 8;28(1):121-129.e4
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western Blot: Eg5 Antibody [GTX30692] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY KIF11 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 μg per lane) were separated by SDS-PAGE and immunoblotted with anti-KIF11.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Eg5 Antibody [GTX30692] - Staining of HeLa cells fixed in 3.5% paraformaldehyde. Cytoplasmic staining during interphase and spindle staining during mitosis.