Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ABIN256417 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Kinesin Family Member 11 (KIF11) antibody
- Antibody type
- Polyclonal
- Antigen
- Modified peptide.
- Description
- Aff - Purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 μL
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Hela cells were treated with 300 uM mimosine for 16 hrs, then placed in complete media (lane 1) or media containing 200 ng/ml nocodazole for an additional 18hrs. Cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit antibody against phosphorylated Eg5 (Thr927). Lane 1, Mimosine only treated Hela cells, Lane 2, mimosine and nocodazole treated Hela. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
