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- References [16]
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- Product number
- NB500-181 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB500-181, RRID:AB_10002881
- Product name
- Rabbit Polyclonal Eg5 Antibody
- Antibody type
- Polyclonal
- Description
- Unpurified.
- Reactivity
- Human, Mouse, Rat, Drosophila, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Differences in Mitotic Spindle Architecture in Mammalian Neural Stem Cells Influence Mitotic Accuracy during Brain Development.
A computational model of the early stages of acentriolar meiotic spindle assembly.
Distribution of Eg5 and TPX2 in mitosis: Insight from CRISPR tagged cells.
Meiotic arrest and spindle defects are associated with altered KIF11 expression in porcine oocytes.
Src family kinase phosphorylation of the motor domain of the human kinesin-5, Eg5.
HDAC Inhibitor-Induced Mitotic Arrest Is Mediated by Eg5/KIF11 Acetylation.
Fbxo30 Regulates Mammopoiesis by Targeting the Bipolar Mitotic Kinesin Eg5.
Kinesin-12 motors cooperate to suppress microtubule catastrophes and drive the formation of parallel microtubule bundles.
Genetic reprogramming of human amniotic cells with episomal vectors: neural rosettes as sentinels in candidate selection for validation assays.
Development of a Drosophila cell-based error correction assay.
Subdiffraction imaging of centrosomes reveals higher-order organizational features of pericentriolar material.
Dual pathway spindle assembly increases both the speed and the fidelity of mitosis.
Dynamic reorganization of Eg5 in the mammalian spindle throughout mitosis requires dynein and TPX2.
Localization and function of Kinesin-5-like proteins during assembly and maintenance of mitotic spindles in Silvetia compressa.
Preventive effects of heregulin-beta1 on macrophage foam cell formation and atherosclerosis.
Prophase microtubule arrays undergo flux-like behavior in mammalian cells.
Vargas-Hurtado D, Brault JB, Piolot T, Leconte L, Da Silva N, Pennetier C, Baffet A, Marthiens V, Basto R
Current biology : CB 2019 Sep 23;29(18):2993-3005.e9
Current biology : CB 2019 Sep 23;29(18):2993-3005.e9
A computational model of the early stages of acentriolar meiotic spindle assembly.
Letort G, Bennabi I, Dmitrieff S, Nedelec F, Verlhac MH, Terret ME
Molecular biology of the cell 2019 Mar 21;30(7):863-875
Molecular biology of the cell 2019 Mar 21;30(7):863-875
Distribution of Eg5 and TPX2 in mitosis: Insight from CRISPR tagged cells.
Mann BJ, Wadsworth P
Cytoskeleton (Hoboken, N.J.) 2018 Dec;75(12):508-521
Cytoskeleton (Hoboken, N.J.) 2018 Dec;75(12):508-521
Meiotic arrest and spindle defects are associated with altered KIF11 expression in porcine oocytes.
Wan X, Zhang Y, Lan M, Pan MH, Tang F, Zhang HL, Ou XH, Sun SC
Environmental and molecular mutagenesis 2018 Dec;59(9):805-812
Environmental and molecular mutagenesis 2018 Dec;59(9):805-812
Src family kinase phosphorylation of the motor domain of the human kinesin-5, Eg5.
Bickel KG, Mann BJ, Waitzman JS, Poor TA, Rice SE, Wadsworth P
Cytoskeleton (Hoboken, N.J.) 2017 Sep;74(9):317-330
Cytoskeleton (Hoboken, N.J.) 2017 Sep;74(9):317-330
HDAC Inhibitor-Induced Mitotic Arrest Is Mediated by Eg5/KIF11 Acetylation.
Nalawansha DA, Gomes ID, Wambua MK, Pflum MKH
Cell chemical biology 2017 Apr 20;24(4):481-492.e5
Cell chemical biology 2017 Apr 20;24(4):481-492.e5
Fbxo30 Regulates Mammopoiesis by Targeting the Bipolar Mitotic Kinesin Eg5.
Liu Y, Wang Y, Du Z, Yan X, Zheng P, Liu Y
Cell reports 2016 May 3;15(5):1111-1122
Cell reports 2016 May 3;15(5):1111-1122
Kinesin-12 motors cooperate to suppress microtubule catastrophes and drive the formation of parallel microtubule bundles.
Drechsler H, McAinsh AD
Proceedings of the National Academy of Sciences of the United States of America 2016 Mar 22;113(12):E1635-44
Proceedings of the National Academy of Sciences of the United States of America 2016 Mar 22;113(12):E1635-44
Genetic reprogramming of human amniotic cells with episomal vectors: neural rosettes as sentinels in candidate selection for validation assays.
Wilson PG, Payne T
PeerJ 2014;2:e668
PeerJ 2014;2:e668
Development of a Drosophila cell-based error correction assay.
Salemi JD, McGilvray PT, Maresca TJ
Frontiers in oncology 2013;3:187
Frontiers in oncology 2013;3:187
Subdiffraction imaging of centrosomes reveals higher-order organizational features of pericentriolar material.
Lawo S, Hasegan M, Gupta GD, Pelletier L
Nature cell biology 2012 Nov;14(11):1148-58
Nature cell biology 2012 Nov;14(11):1148-58
Dual pathway spindle assembly increases both the speed and the fidelity of mitosis.
Kaseda K, McAinsh AD, Cross RA
Biology open 2012 Jan 15;1(1):12-8
Biology open 2012 Jan 15;1(1):12-8
Dynamic reorganization of Eg5 in the mammalian spindle throughout mitosis requires dynein and TPX2.
Gable A, Qiu M, Titus J, Balchand S, Ferenz NP, Ma N, Collins ES, Fagerstrom C, Ross JL, Yang G, Wadsworth P
Molecular biology of the cell 2012 Apr;23(7):1254-66
Molecular biology of the cell 2012 Apr;23(7):1254-66
Localization and function of Kinesin-5-like proteins during assembly and maintenance of mitotic spindles in Silvetia compressa.
Peters NT, Miller AC, Kropf DL
BMC research notes 2009 Jun 15;2:106
BMC research notes 2009 Jun 15;2:106
Preventive effects of heregulin-beta1 on macrophage foam cell formation and atherosclerosis.
Xu G, Watanabe T, Iso Y, Koba S, Sakai T, Nagashima M, Arita S, Hongo S, Ota H, Kobayashi Y, Miyazaki A, Hirano T
Circulation research 2009 Aug 28;105(5):500-10
Circulation research 2009 Aug 28;105(5):500-10
Prophase microtubule arrays undergo flux-like behavior in mammalian cells.
Ferenz NP, Wadsworth P
Molecular biology of the cell 2007 Oct;18(10):3993-4002
Molecular biology of the cell 2007 Oct;18(10):3993-4002
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Eg5 Antibody [NB500-181] - Human Eg5 rescues depletion of the Drosophila kinesin-5 family member Klp61F. Western blot analysis of cell lysates prepared from induced and uninduced; control and Klp61F RNAi-treated cells blotted with anti-Eg5 to monitor induction of Eg5-mCherry, anti-Klp61F to confirm depletion of the motor and anti-alpha-tubulin as a loading control. Cells depleted of Klp61F and with Eg5-mCherry induced are referred to as RepEg5 cells. Image collected and cropped by CiteAb from the following publication (http://journal.frontiersin.org/article/10.3389/fonc.2013.00187/abstract), licensed under a CC-BY licence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Eg5 Antibody [NB500-181] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY KIF11 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Eg5.
