Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- ABIN953071 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Kinesin Family Member 11 (KIF11) (N-Term), (AA 109-139) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 109~139 amino acids from the N-terminal region of human KIF11
- Description
- Protein A column, followed by peptide affinity purification
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- N-Term,AA 109-139
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Mutations in the human kinesin Eg5 that confer resistance to monastrol and S-trityl-L-cysteine in tumor derived cell lines.
ATP hydrolysis in Eg5 kinesin involves a catalytic two-water mechanism.
Tcherniuk S, van Lis R, Kozielski F, Skoufias DA
Biochemical pharmacology 2010 Mar 15;79(6):864-72
Biochemical pharmacology 2010 Mar 15;79(6):864-72
ATP hydrolysis in Eg5 kinesin involves a catalytic two-water mechanism.
Parke CL, Wojcik EJ, Kim S, Worthylake DK
The Journal of biological chemistry 2010 Feb 19;285(8):5859-67
The Journal of biological chemistry 2010 Feb 19;285(8):5859-67
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of KIF11 Antibody (N-term) Cat.-No AP52363PU-N in CHO cell line lysates (35ug/lane). KIF11 (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human lung carcinoma reacted with KIF11 Antibody (N-term) Cat.-No AP52363PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Flow cytometry analysis of Hela cells using KIF11 Antibody (N-term) Cat.-No AP52363PU-N (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.