HPA023447

antibody from Atlas Antibodies

Targeting: DDX42 RHELP, RNAHP, SF3b125, SF3B8

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA023447

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA023447, RRID:AB_1847560

Product name

Anti-DDX42

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

HGETGNRHSDSPRHGDGGRHGDGYRHPESSSRHTD
GHRHGENRHGGSAGRHGENRGANDGRNGESRKEAF
NRESKMEPKMEPKVDSSKMDKVDSKTDKTADGFAV
PEPPKRKKSR


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human fallopian tube shows moderate nuclear positivity in glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver shows weak nuclear positivity in hepatocytes.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human fallopian tube shows moderate nuclear positivity in glandular cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human tonsil shows moderate nuclear positivity.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.

Sample type

HUMAN