HPA023571

DDX42 antibody from Atlas Antibodies

RHELP, RNAHP, SF3b125, SF3B8

Validated within the Antibodypedia Validation Initative

Western blot

Supportive data in Antibodypedia

Immunocytochemistry

Enhanced validation

Immunohistochemistry

Supportive data in Antibodypedia

Provider product page for HPA023571

Antibody Data

Product number

HPA023571

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA023571, RRID:AB_1847561

Product name

Anti-DDX42

Provider product page

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

FGATSSSSGFGKSAPPQLPSFYKIGSKRANFDEEN
AYFEDEEEDSSNVDLPYIPAENSPTRQQFHSKPVD
SDSDDDPLEAFMAEVEDQAARDMKRLEEKDKERKN
VKGIRDD


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.

Sample type

HUMAN

Enhanced validation

Submitted by

55af80e3e0991

Enhanced method

Genetic validation

Main image

Experimental details

Confocal images of immunofluorescently stained human U-2 OS cells.The protein DDX42 is shown in green and the microtubules in red. The image to the left show cells transfected with control siRNA and the image to the right show cells where DDX42has been downregulated with specific siRNA.

Sample type

U-2 OS cells

Primary Ab dilution

1:46

Secondary Ab

Secondary Ab

Secondary Ab dilution

1:800

Knockdown/Genetic Approaches Application

Immunocytochemistry

KD Reagent Type

siRNA

Downregulation

>75%

KD Reagent Provider

Thermo Fisher Scientific

KD Reagent Prod no

s22329

KD Reagent Prod page

KD Reagent Prod page

KD Reagent Prod no 2

s22328

KD Reagent Page 2

KD Reagent Page 2

KD Reagent Prod no 3

s22330

KD Reagent Page 3

KD Reagent Page 3

Antibody Lot Number

R11046

Appendix

55b89db836424.pdf

Show more

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human fallopian tube shows moderate nuclear positivity in glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human parathyroid gland shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas shows low expression as expected.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human parathyroid gland and pancreas tissues using Anti-DDX42 antibody. Corresponding DDX42 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Show more