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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [2]
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- Product number
- HPA023571 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA023571, RRID:AB_1847561
- Product name
- Anti-DDX42
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human DDX42, Gene description: DEAD (Asp-Glu-Ala-Asp) box helicase 42, Alternative Gene Names: RHELP, RNAHP, SF3b125, SF3B8, Validated applications: WB, IHC, ICC, Uniprot ID: Q86XP3, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Nuclear bodies formed by polyQ-ataxin-1 protein are liquid RNA/protein droplets with tunable dynamics
Zhang S, Hinde E, Parkyn Schneider M, Jans D, Bogoyevitch M
Scientific Reports 2020;10(1)
Scientific Reports 2020;10(1)
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Enhanced validation
Supportive validation
- Submitted by
- 55af80e3e0991
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Confocal images of immunofluorescently stained human U-2 OS cells.The protein DDX42 is shown in green and the microtubules in red. The image to the left show cells transfected with control siRNA and the image to the right show cells where DDX42has been downregulated with specific siRNA.
- Sample type
- U-2 OS cells
- Primary Ab dilution
- 1:46
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1:800
- Knockdown/Genetic Approaches Application
- Immunocytochemistry
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.
- Sample type
- Human
