PA5-54828

antibody from Invitrogen Antibodies

Targeting: DDX42 RHELP, RNAHP, SF3b125, SF3B8

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-54828

Provider

Invitrogen Antibodies

Product name

DDX42 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: FGATSSSSGF GKSAPPQLPS FYKIGSKRAN FDEENAYFED EEEDSSNVDL PYIPAENSPT RQQFHSKPVD SDSDDDPLEA FMAEVEDQAA RDMKRLEEKD KERKNVKGIR DD

Concentration

0.1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DDX42 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4. Samples were probed using a DDX42 Polyclonal Antibody (Product # PA5-54828).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DDX42 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a DDX42 Polyclonal Antibody (Product # PA5-54828).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of DDX42 in human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a DDX42 Polyclonal Antibody (Product # PA5-54828).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of DDX42 in human cell line U-2 OS using a DDX42 Polyclonal Antibody (Product # PA5-54828) shows localization to nuclear speckles.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of DDX42 in human fallopian tube using a DDX42 Polyclonal Antibody (Product # PA5-54828) shows moderate nuclear positivity in glandular cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of DDX42 in human pancreas using DDX42 Polyclonal Antibody (Product # PA5-54828) shows low expression as expected.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of DDX42 in human parathyroid gland using DDX42 Polyclonal Antibody (Product # PA5-54828) shows high expression.