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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
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- Product number
- PA5-55273 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-DDX42 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: TAGVVQEEEE DNLEYDSDGN PIAPTKKIID PLPPIDHSEI DYPPFEKNFY NEHEEITNLT PQQLIDLRHK LNLRVSGAAP PRPGSSFAHF GFDEQLMHQI RKSEYTQPTP IQCQG Highest antigen sequence identity to the following orthologs: Mouse - 99%, Rat - 99%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.05 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DDX42 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a DDX42 Polyclonal Antibody (Product # PA5-55273).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DDX42 in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a DDX42 Polyclonal Antibody (Product # PA5-55273). Remaining relative intensity is presented.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of DDX42 in human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a DDX42 Polyclonal Antibody (Product # PA5-55273).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of DDX42 in human cell line U-251 MG using a DDX42 Polyclonal Antibody (Product # PA5-55273) shows localization to nuclear speckles.
