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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00079039-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00079039-M01, RRID:AB_538218
- Product name
- Mouse Monoclonal DDX54 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. DDX54 - DEAD (Asp-Glu-Ala-Asp) box polypeptide 54
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DDX54 Antibody (2H6) [H00079039-M01] - Analysis of DDX54 expression in transfected 293T cell line by DDX54 monoclonal antibody (M01), clone 2H6.Lane 1: DDX54 transfected lysate(98.6 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DDX54 Antibody (2H6) [H00079039-M01] - DDX54 monoclonal antibody (M01), clone 2H6 Analysis of DDX54 expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: DDX54 Antibody (2H6) [H00079039-M01] - Detection limit for recombinant GST tagged DDX54 is 0.03 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: DDX54 Antibody (2H6) [H00079039-M01] - Analysis of monoclonal antibody to DDX54 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DDX54 Antibody (2H6) [H00079039-M01] - Analysis of monoclonal antibody to DDX54 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. Antibody concentration 3 ug/ml.
