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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- AP09396PU-S - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Anti-Pogz Antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Anti-Pogz Antibody, Aff - Purified
- Reactivity
- Human, Mouse, Rat, Canine, Chicken/Avian
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 25ul
- Concentration
- 0.564 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot using affinity purified anti-Pogz antibody shows detection of Pogz protein (arrowhead) in adult mouse thymus and spleen tissue lysate (Panel A). The lower molecular weight bands may be cross reactive proteins. Pre-incubation of antibody with the immunizing peptide blocks specific antibody reactivity. Primary antibody was used at 1:3,000.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot using affinity purified anti-Pogz antibody shows detection (Panel A) of Pogz protein (arrowhead) in brain whole cell lysates from wild type (WT) adult mice (lanes 1-3). No band is seen in similar lysates from knockout (KO) mice (lanes 4-6). Pre-incubation of antibody with the immunizing peptide blocks specific antibody reactivity (Panel B). Primary antibody was used at 1:3,000.
- Validation comment
- WB
