PA5-22070

antibody from Invitrogen Antibodies

Targeting: PNLIP PL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-22070

Provider

Invitrogen Antibodies

Product name

PNLIP Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, A549, HCT116.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Pancreatic Lipase using 30 µg of 293T lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Pancreatic Lipase polyclonal antibody (Product # PA5-22070) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of PNLIP was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a PNLIP Polyclonal Antibody (Product # PA5-22070) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PNLIP Polyclonal Antibody detects PNLIP protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract.10 % SDS-PAGE. PNLIP Polyclonal Antibody (Product # PA5-22070) dilution: 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Pancreatic Lipase in paraformaldehyde-fixed A549 cells using a Pancreatic Lipase polyclonal antibody (Product # PA5-22070) at a 1:200 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of paraformaldehyde-fixed A549, using Pancreatic Lipase antibody (Product # PA5-22070) at 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded SCM-1 xenograft, using Pancreatic Lipase (Product # PA5-22070) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.