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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-54143 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ABCF2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: KEAAKARQRP RKGHEENGDV VTEPQVAEKN EANGRETTEV DLLTKELEDF EMKKAAARAV TGVLASHPNS TDVHIINLSL TFHGQELLSD TKLELNSGRR YGLIGLNGIG KS Highest antigen sequence identity to the following orthologs: Mouse - 93%, Rat - 97%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.4 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ABCF2 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using an ABCF2 Polyclonal Antibody (Product # PA5-54143).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ABCF2 using ABCF2 Polyclonal Antibody (Product # PA5-54143) (A) shows similar pattern to an independent ABCF2 Polyclonal Antibody (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of ABCF2 in human cell line A-431 using an ABCF2 Polyclonal Antibody (Product # PA5-54143) shows localization to cytosol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ABCF2 in human testis and liver tissues using ABCF2 Polyclonal Antibody (Product # PA5-54143). Corresponding ABCF2 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ABCF2 in human liver using ABCF2 Polyclonal Antibody (Product # PA5-54143) shows low expression as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ABCF2 in human testis using ABCF2 Polyclonal Antibody (Product # PA5-54143) shows high expression.
