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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- H00010061-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00010061-M01, RRID:AB_536530
- Product name
- Mouse Monoclonal ABCF2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ABCF2 - ATP-binding cassette, sub-family F (GCN20), member 2
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ABCF2 Antibody (1D11) [H00010061-M01] - Analysis of ABCF2 over-expressed 293 cell line, cotransfected with ABCF2 Validated Chimera RNAi ( Cat # H00010061-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ABCF2 monoclonal antibody (M01), clone 1D11 (Cat # H00010061-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ABCF2 Antibody (1D11) [H00010061-M01] - Analysis of ABCF2 expression in transfected 293T cell line by ABCF2 monoclonal antibody (M01), clone 1D11.Lane 1: ABCF2 transfected lysate(68.53 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ABCF2 Antibody (1D11) [H00010061-M01] - ABCF2 monoclonal antibody (M01), clone 1D11 Analysis of ABCF2 expression in Hela.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: ABCF2 Antibody (1D11) [H00010061-M01] - Detection limit for recombinant GST tagged ABCF2 is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: ABCF2 Antibody (1D11) [H00010061-M01] - Analysis of ABCF2 transfected lysate using anti-ABCF2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ABCF2 monoclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ABCF2 Antibody (1D11) [H00010061-M01] - Analysis of monoclonal antibody to ABCF2 on formalin-fixed paraffin-embedded human prostate. Antibody concentration 3 ug/ml.
