PA5-118208

antibody from Invitrogen Antibodies

Targeting: PSMC5 p45, p45/SUG, S8, SUG-1, SUG1, TBP10, TRIP1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-118208

Provider

Invitrogen Antibodies

Product name

PSMC5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using PSMC5 Polyclonal Antibody (Product # PA5-118208) at 1:500 dilution. Lane A: HeLa Whole Cell Lysate, Lane B: HepG2 Whole Cell Lysate, Lane C: 293T Whole Cell Lysate, Lane D: A431 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 46 kDa. Observed band size: 46 kDa. (We are unsure of the identity of these extra bands).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using PSMC5 Polyclonal Antibody (Product # PA5-118208) at 1:500 dilution. Lane A: HeLa Whole Cell Lysate, Lane B: HepG2 Whole Cell Lysate, Lane C: 293T Whole Cell Lysate, Lane D: A431 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 46 kDa. Observed band size: 46 kDa. (We are unsure of the identity of these extra bands).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human PSMC5 in human brain with PSMC5 Polyclonal Antibody (Product # PA5-118208, 1:100 dilution, formalin-fixed paraffin embedded sections).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PSMC5 Immunoprecipitation using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 4 µL with PSMC5 Polyclonal Antibody (Product # PA5-118208) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: PSMC5 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1,000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 46 kDa. Observed band size: 46 kDa.