PA5-76010

antibody from Invitrogen Antibodies

Targeting: AGPAT2 BSCL, LPAAT-beta

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-76010

Provider

Invitrogen Antibodies

Product name

AGPAT2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Ferroptosis response segregates small cell lung cancer (SCLC) neuroendocrine subtypes.

Bebber CM, Thomas ES, Stroh J, Chen Z, Androulidaki A, Schmitt A, Höhne MN, Stüker L, de Pádua Alves C, Khonsari A, Dammert MA, Parmaksiz F, Tumbrink HL, Beleggia F, Sos ML, Riemer J, George J, Brodesser S, Thomas RK, Reinhardt HC, von Karstedt S
Nature communications 2021 Apr 6;12(1):2048

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of AGPAT2 in HeLa cells. Samples were incubated with AGPAT2 polyclonal antibody (Product # PA5-76010) followed by DAPI (nuclear staining).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 4 non-NE/NE SCLC subtypes undergo lipid metabolism remodeling. a Heatmap showing the representation of mono-oxidized phospholipid species (PE phosphatidylethanolamine; PC phosphatidylcholine) in 181.5 stickers as compared to 181.5 floaters treated with either DMSO or RSL3 [1 uM] for 5 h and then subjected to lipidomics. Samples for each condition ( n = 5) were averaged and normalized to the cell number (2.5 x 10 6 ). Each lipid species was normalized to levels detected in floaters +DMSO. One representative out of two independent experiments is shown. Heatmap color code indicates normalized lipid species levels of each sample. b - e 181.5 stickers floaters ( n = 5 samples) as compared to 181.5 floaters ( n = 5 samples) were analyzed for basal diacylglycerol (DAG) and ether-linked lipids by mass spectrometry. Lipid content was normalized to infused protein for each condition and replicate. Individual PUFAs (4 double bonds or more) are plotted. f RNA was isolated from three stickers / floaters lines (RP181.5; RP246.7; BYC5.1), respective cDNA obtained and qPCR performed for the indicated transcripts. g RP181.5 were subjected to the indicated siRNA-mediated knockdowns for 72 h and then treated with RSL3 [1 uM] for an additional 24 h. DRAQ7 [0.1 uM] was added to all wells to visualize dead cells. Images were acquired every 2 h using the IncuCyte S3 bioimaging platform. Dead cells/image are normalized to cell confluence at the beginning of RSL3 treatment. h Representative west