H00006009-M05

antibody from Abnova Corporation

Targeting: RHEB RHEB2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00006009-M05

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00006009-M05, RRID:AB_566131

Product name

RHEB monoclonal antibody (M05), clone 1E12

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant RHEB.

Antigen sequence

MPQSKSRKIAILGYRSVGKSSLTIQFVEGQFVDSY
DPTIENTFTKLITVNGQEYHLQLVDTAGQDEYSIF
PQTYSIDINGYILVYSVTSIKSFEVIKVIHGKLLD
MVGKVQIPIMLVGNKKDLHMERVISYEEGKALAES
WNAAFLESSAKENQTAVDVFRRIILEAEKMDGAAS
QGKSSCSVM


Isotype

IgG

Antibody clone number

1E12

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of RHEB expression in transfected 293T cell line by RHEB monoclonal antibody (M05), clone 1E12.Lane 1: RHEB transfected lysate(20.5 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged RHEB is approximately 0.1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol