H00006009-M01

antibody from Abnova Corporation

Targeting: RHEB RHEB2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00006009-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00006009-M01, RRID:AB_1112097

Product name

RHEB monoclonal antibody (M01), clone 2C11

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant RHEB.

Antigen sequence

MPQSKSRKIAILGYRSVGKSSLTIQFVEGQFVDSY
DPTIENTFTKLITVNGQEYHLQLVDTAGQDEYSIF
PQTYSIDINGYILVYSVTSIKSFEVIKVIHGKLLD
MVGKVQIPIMLVGNKKDLHMERVISYEEGKALAES
WNAAFLESSAKENQTAVDVFRRIILEAEKMDGAAS
QGKSSCSVM


Isotype

IgG

Antibody clone number

2C11

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

p53 Deletion or Hotspot Mutations Enhance mTORC1 Activity by Altering Lysosomal Dynamics of TSC2 and Rheb.

Agarwal S, Bell CM, Taylor SM, Moran RG
Molecular cancer research : MCR 2016 Jan;14(1):66-77

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Spatial control of the TSC complex integrates insulin and nutrient regulation of mTORC1 at the lysosome.

Menon S, Dibble CC, Talbott G, Hoxhaj G, Valvezan AJ, Takahashi H, Cantley LC, Manning BD
Cell 2014 Feb 13;156(4):771-85

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

RHEB monoclonal antibody (M01), clone 2C11. Western Blot analysis of RHEB expression in Raw 264.7 ( Cat # L024V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged RHEB is approximately 3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol