PA5-20129

antibody from Invitrogen Antibodies

Targeting: RHEB RHEB2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-20129

Provider

Invitrogen Antibodies

Product name

RHEB Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

A suggested positive control is mouse brain tissue lysate. PA5-20129 can be used with blocking peptide PEP-0247.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

References

Submitted references

FMRP S499 is phosphorylated independent of mTORC1-S6K1 activity.

Bartley CM, O'Keefe RA, Bordey A
PloS one 2014;9(5):e96956

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MEK-ERK1/2-dependent FLNA overexpression promotes abnormal dendritic patterning in tuberous sclerosis independent of mTOR.

Zhang L, Bartley CM, Gong X, Hsieh LS, Lin TV, Feliciano DM, Bordey A
Neuron 2014 Oct 1;84(1):78-91

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of mouse brain cell lysate using a Rheb polyclonal antibody (Product # PA5-20129) at (A) 1, (B) 2, and (C) 4 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Rheb in mouse brain cell lysate with RHEB Polyclonal Antibody (Product # PA5-20129) at (A) 1, (B) 2, and (C) 4 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Rheb in mouse brain cell lysate with RHEB Polyclonal Antibody (Product # PA5-20129) at (A) 1, (B) 2, and (C) 4 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RHEB was performed by loading whole-cell lysates of mouse tissue samples. RHEB was detected at approximately ~20 kDa by using a RHEB polyclonal Antibody (Product # PA5-20129) at a concentration of 1.0 µg/mL in Blocking Buffer overnight at 4°C on a rocking platform, followed by a 1-hour room-temperature incubation of a donkey anti-rabbit IgG antibody at a dilution of 1:5,000. Data courtesy of Thermo Scientific KOL Program.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of mouse brain cells using a Rheb polyclonal antibody (Product # PA5-20129) at a 20 µg/mL dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of Rheb in Mouse Brain cells with RHEB Polyclonal Antibody (Product # PA5-20129) at 20 µg/mL.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of Rheb in mouse brain tissue with RHEB Polyclonal Antibody (Product # PA5-20129) at 2 µg/mL.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 Hyperactive mTORC1-S6K1 does not alter pFMRP S499. ( A ) Cre expression and genetic recombination were verified by region-specific tdTomato expression (note tdTomato in telencephalon, Tel, but not cerebellum, Cb). Genotypes were confirmed by PCR: wild type mice (WT) have two wild type alleles (wt, 295bp amplicon), heterozygous mice (Het) have one wt and one mutant allele (mut, 370bp amplicon), and conditional mice have one floxed allele (fl, 480bp amplicon) with either a wt or mut allele. Asterisks indicate nonspecific bands ( B ) Immunoblotting of cortical lysates from P7 Tsc1 mice of different genotypes listed under the blots. ( C ) There is no statistical difference in pFMRP:tFMRP among genotypes despite significantly increased pS6:S6 in Tsc1 fl/mut vs. Tsc1 WT . pFMRP:tFMRP was quantified by unpaired, one-way ANOVA. pS6:S6 was quantified using unpaired, nonparametric one-sided Mann-Whitney test (** = p