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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-27588 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RAP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HeLa, HepG2, HCT116, mouse brain. Predicted reactivity: Mouse (86%), Rat (86%), Chimpanzee (98%), Bovine (90%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.61 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RAP1 using 30 µg of A) HepG2 and B) HCT116 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a RAP1 polyclonal antibody (Product # PA5-27588) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RAP1 Polyclonal Antibody (Product # PA5-27588). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 10% SDS PAGE. RAP1 Polyclonal Antibody (Product # PA5-27588) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RAP1 Polyclonal Antibody (Product # PA5-27588). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with RAP1 Polyclonal Antibody (Product # PA5-27588) diluted at 1:3,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RAP1 Polyclonal Antibody detects RAP1 protein at nucleus and cytoplasm by immunofluorescent analysis. Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RAP1 protein stained by RAP1 Polyclonal Antibody (Product # PA5-27588) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SCM1 xenograft, using RAP1 (Product # PA5-27588) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RAP1 antibody immunoprecipitates RAP1 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : Control with 3 µg of pre-immune rabbit IgG B : Immunoprecipitation of RAP1 by 3 µg of RAP1 antibody (Product # PA5-27588) 10% SDS-PAGE The immunoprecipitated RAP1 protein was detected by RAP1 antibody (Product # PA5-27588) diluted at 1:1,000. Anti-rabbit IgG (HRP) was used as a secondary reagent.
