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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-78551 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GNB4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: Jurkat, Raji, NCI-H929 Predicted Reactivity: Mouse (100%), Rat (100%), Xenopus laevis (100%), Rhesus Monkey (100%), Bovine (100%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.93 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Gnb4 in whole cell lysate using 30 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with Gnb4 polyclonal antibody (Product # PA5-78551) using a dilution of 1:1000 followed by HRP-conjugated anti-rabbit IgG.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GNB4 Polyclonal Antibody detects G protein beta protein at cell membrane and cytoplasm by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: G protein beta protein stained by GNB4 Polyclonal Antibody (Product # PA5-78551) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of GNB4 was performed in paraffin-embedded human colon cancer tissue using GNB4 Polyclonal Antibody (Product # PA5-78551) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of GNB4 was performed in paraffin-embedded human ovarian cancer tissue using GNB4 Polyclonal Antibody (Product # PA5-78551) at a dilution of 1:500.
