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Immunocytochemistry
Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- HPA064683 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA064683, RRID:AB_2685320
- Product name
- Anti-TBX5
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human TBX5, Gene description: T-box 5, Alternative Gene Names: HOS, Validated applications: ICC, ChIP, Uniprot ID: Q99593, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Recapitulating porcine cardiac development in vitro: from expanded potential stem cell to embryo culture models
Rawat H, Kornherr J, Zawada D, Bakhshiyeva S, Kupatt C, Laugwitz K, Bähr A, Dorn T, Moretti A, Nowak-Imialek M
Frontiers in Cell and Developmental Biology 2023;11
Frontiers in Cell and Developmental Biology 2023;11
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Supportive validation
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- Atlas Antibodies (provider)
- Main image
- Experimental details
- ChIP-Exo-Seq composite graph for Anti-TBX5 (HPA064683, Lot 000021600) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.
