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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [2]
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- Product number
- NBP1-33715 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-33715, RRID:AB_2200707
- Product name
- Rabbit Polyclonal TBX5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TBX5 Antibody [NBP1-33715] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE; antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TBX5 Antibody [NBP1-33715] - A. rat brain lysate/extract.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TBX5 Antibody [NBP1-33715] - HeLa whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TBX5 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TBX5 Antibody [NBP1-33715] - Rat thyroid gland. TBX5 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TBX5 Antibody [NBP1-33715] - Rat ovary. TBX5 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
