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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- NBP1-77981 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-77981, RRID:AB_11018472
- Product name
- Rabbit Polyclonal Rad9 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This affinity purified antibody is directed against the phosphorylated form of yeast Rad9 at the pS1260 residue.
- Reactivity
- Yeast
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.41 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: Rad9 [p Ser1260] Antibody [NBP1-77981] - This antibody was used at a 1:200 dilution incubated overnight at 4C. Lanes were loaded with 50 ug each of recombinant GST fusion protein containing S. cerevisiae Rad9 (aa 991-1309 ~60 kDa) on a 4-20% Criterion gel for SDS-PAGE as follows: Lane 1 - non-phosphorylated wild type yeast Rad9, Lane 2 - in vitro phosphorylated wild type yeast Rad9, Lane 3 - non-phosphorylated S1129A/S1260A double mutant Rad9, Lane 4 - in vitro phosphorylated S1129A/S1260A double mutant. Phosphorylation of Rad9 was by treatment with ATP and Rad53 kinase. Rad53 kinase autophosphorylates and appears cross reactive as it is detected on the blot as 90 and 110 kDa bands (asterisk). Detection occurred using a 1:5,000 dilution of IRDye (TM) 800 conjugated Donkey anti-Rabbit IgG for 1h at room temperature.
