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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- NBP1-77978 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-77978, RRID:AB_11041363
- Product name
- Rabbit Polyclonal Rad9 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This affinity purified antibody is directed against an internal sequence of yeast Rad9 at the S1129 residue. Antiserum was purified against the immunizing peptide.
- Reactivity
- Yeast
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: Rad9 Antibody [NBP1-77978] - Used at a 1:200 dilution incubated 8 hr at room temperature to detect Rad9 by Western blot. Lanes were loaded with 50 ng each of recombinant GST fusion protein containing S. cerevisiae Rad9 (aa 991-1309 ~60 kDa) on a 4-20% Criterion gel for SDS-PAGE as follows: Lane 1 - non-phosphorylated wild type yeast Rad9, Lane 2 - in vitro phosphorylated wild type yeast Rad9, Lane 3 - non-phosphorylated S1129A/S1260A double mutant Rad9, Lane 4 - in vitro phosphorylated S1129A/S1260A double mutant.
