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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB0588 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0588, RRID:AB_982854
- Product name
- RAD9A (phospho S387) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of RAD9A.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Accumulation of hRad9 protein in the nuclei of nonsmall cell lung carcinoma cells.
The human Rad9 checkpoint protein stimulates the carbamoyl phosphate synthetase activity of the multifunctional protein CAD.
The human Rad9-Rad1-Hus1 checkpoint complex stimulates flap endonuclease 1.
Maniwa Y, Yoshimura M, Bermudez VP, Yuki T, Okada K, Kanomata N, Ohbayashi C, Hayashi Y, Hurwitz J, Okita Y
Cancer 2005 Jan 1;103(1):126-32
Cancer 2005 Jan 1;103(1):126-32
The human Rad9 checkpoint protein stimulates the carbamoyl phosphate synthetase activity of the multifunctional protein CAD.
Lindsey-Boltz LA, Wauson EM, Graves LM, Sancar A
Nucleic acids research 2004;32(15):4524-30
Nucleic acids research 2004;32(15):4524-30
The human Rad9-Rad1-Hus1 checkpoint complex stimulates flap endonuclease 1.
Wang W, Brandt P, Rossi ML, Lindsey-Boltz L, Podust V, Fanning E, Sancar A, Bambara RA
Proceedings of the National Academy of Sciences of the United States of America 2004 Nov 30;101(48):16762-7
Proceedings of the National Academy of Sciences of the United States of America 2004 Nov 30;101(48):16762-7
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The RAD9A (phospho S387) polyclonal antibody (Cat # PAB0588) is used in Western blot to detect Phospho-RAD9A-S387 in Y-79 (left) and Hela (right) cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with RAD9A (phospho S387) polyclonal antibody (Cat # PAB0588) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
