TA500435

antibody from OriGene

Targeting: RAD9A RAD9

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500435

Provider

OriGene

Proper citation

OriGene Cat#TA500435, RRID:AB_2177717

Product name

Anti-RAD9A (RAD9) mouse monoclonal antibody, clone OTI5D9 (formerly 5D9)

Antibody type

Monoclonal

Description

Anti-RAD9A (RAD9) mouse monoclonal antibody, clone OTI5D9 (formerly 5D9)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

RAD9A

Isotype

IgG

Antibody clone number

OTI5D9

Vial size

100 µl

Concentration

1.2 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAD9A (RC204439, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAD9A.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-anti-RAD9Amonoclonal antibody.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-RAD9Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500435, Dilution 1:50)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-RAD9Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500435, Dilution 1:50)

Validation comment

IHC