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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Product number
- ABIN358201 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-RAD9 Homolog A (S. Pombe) (RAD9A) (pSer277) antibody
- Antibody type
- Polyclonal
- Description
- Protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- pSer277
- Vial size
- 0.4 mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Accumulation of hRad9 protein in the nuclei of nonsmall cell lung carcinoma cells.
The human Rad9 checkpoint protein stimulates the carbamoyl phosphate synthetase activity of the multifunctional protein CAD.
The human Rad9/Rad1/Hus1 damage sensor clamp interacts with DNA polymerase beta and increases its DNA substrate utilisation efficiency: implications for DNA repair.
The human Rad9-Rad1-Hus1 checkpoint complex stimulates flap endonuclease 1.
Rad9 protects cells from topoisomerase poison-induced cell death.
Maniwa Y, Yoshimura M, Bermudez VP, Yuki T, Okada K, Kanomata N, Ohbayashi C, Hayashi Y, Hurwitz J, Okita Y
Cancer 2005 Jan 1;103(1):126-32
Cancer 2005 Jan 1;103(1):126-32
The human Rad9 checkpoint protein stimulates the carbamoyl phosphate synthetase activity of the multifunctional protein CAD.
Lindsey-Boltz LA, Wauson EM, Graves LM, Sancar A
Nucleic acids research 2004;32(15):4524-30
Nucleic acids research 2004;32(15):4524-30
The human Rad9/Rad1/Hus1 damage sensor clamp interacts with DNA polymerase beta and increases its DNA substrate utilisation efficiency: implications for DNA repair.
Toueille M, El-Andaloussi N, Frouin I, Freire R, Funk D, Shevelev I, Friedrich-Heineken E, Villani G, Hottiger MO, Hübscher U
Nucleic acids research 2004;32(11):3316-24
Nucleic acids research 2004;32(11):3316-24
The human Rad9-Rad1-Hus1 checkpoint complex stimulates flap endonuclease 1.
Wang W, Brandt P, Rossi ML, Lindsey-Boltz L, Podust V, Fanning E, Sancar A, Bambara RA
Proceedings of the National Academy of Sciences of the United States of America 2004 Nov 30;101(48):16762-7
Proceedings of the National Academy of Sciences of the United States of America 2004 Nov 30;101(48):16762-7
Rad9 protects cells from topoisomerase poison-induced cell death.
Loegering D, Arlander SJ, Hackbarth J, Vroman BT, Roos-Mattjus P, Hopkins KM, Lieberman HB, Karnitz LM, Kaufmann SH
The Journal of biological chemistry 2004 Apr 30;279(18):18641-7
The Journal of biological chemistry 2004 Apr 30;279(18):18641-7
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