Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-21275 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RAD9 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, HeLa, C2C12, Rat2. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Rad9 using 30 µg Rat2 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Rad9 polyclonal antibody (Product # PA5-21275) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Rad9 using 30 µg of A) A431 and B) HeLa lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Rad9 polyclonal antibody (Product # PA5-21275) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RAD9 was performed by separating 30 µg of various whole cell lysates by 10 % SDS-PAGE. Proteins were transferred to a membrane and probed with a RAD9 Polyclonal Antibody (Product # PA5-21275) at a dilution of 1:1000. A. 293T, B. A431, C. HeLa, D. HepG2.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RAD9 was performed by separating 30 µg of various whole cell lysates by 10 % SDS-PAGE. Proteins were transferred to a membrane and probed with a RAD9 Polyclonal Antibody (Product # PA5-21275) at a dilution of 1:500. A. Neuro2A, B. GL261, C. C8D30, D. NIH-3T3, E. BCL-1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RAD9 was performed by separating 30 µg of PC-12 whole cell lysates by 10 % SDS-PAGE. Proteins were transferred to a membrane and probed with a RAD9 Polyclonal Antibody (Product # PA5-21275) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Rad9 in paraformaldehyde-fixed HeLa cells using a Rad9 polyclonal antibody (Product # PA5-21275) at a 1:100 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Rad9 antibody (Product # PA5-21275) at 1:100 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RAD9 Polyclonal Antibody detects RAD9A protein at nucleus on HBL435 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HBL435 xenograft. RAD9 Polyclonal Antibody (Product # PA5-21275) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.