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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AP11314PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP11314PU-N, RRID:AB_1770851
- Product name
- anti RAD9A (BH3 Domain)
- Antibody type
- Polyclonal
- Antigen
- This antibody was developed against a synthetic peptide corresponding within amino acid residues 1-100 of human Rad9.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- LEFT: Western blot analysis of anti-Rad9 BH3 domain Pab (Cat. #AP11314PU-N) in HL-60 cell lysate. Rad9 BH3 domain (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. RIGHT: Western blot analysis of Rad9(arrow) using rabbit polyclonal Rad9 BH3 domain Pab (Cat. #AP11314PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Rad9 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
