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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB20358 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB20358, RRID:AB_10965684
- Product name
- FLII polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against recombinant FLII.
- Antigen sequence
KDSAQDDQAKQVLKGMSDVAQEKNKKQEESADARA
PSGKVRRWDQGLEKPRLDYSEFFTEDVGQLPGLTI
WQIENFVPVLVEEAFHGKFYEADCYIVLKTFLDDS
GSLNWEIYYWIGGEATLDKKACSAI- Isotype
- IgG
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with FLII polyclonal antibody (Cat # PAB20358) at 1:250-1:500 dilution.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of cell lysates with FLII polyclonal antibody (Cat # PAB20358) at 1:250-1:500 dilution.Lane 1 : NIH/3T3Lane 2 : NBT-II
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line U-2 OS with FLII polyclonal antibody (Cat # PAB20358) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli, cytoplasm, centrosome.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human skeletal muscle with FLII polyclonal antibody (Cat # PAB20358) shows strong cytoplasmic positivity in myocytes at 1:20-1:50 dilution.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
