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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [4]
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- Product number
- PA5-21735 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FLII Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, H1299, HeLaS3, Molt-4, Raji, mouse brain. Predicted reactivity: Mouse (96%), Rat (96%), Zebrafish (81%), Chicken (87%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.88 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Flightless I Negatively Regulates Macrophage Surface TLR4, Delays Early Inflammation, and Impedes Wound Healing.
Mills SJ, Ahangar P, Thomas HM, Hofma BR, Murray RZ, Cowin AJ
Cells 2022 Jul 13;11(14)
Cells 2022 Jul 13;11(14)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using FLII Polyclonal Antibody (Product # PA5-21735). Sample (30 µg whole cell lysate). A: Raji.5% SDS PAGE. FLII Polyclonal Antibody (Product # PA5-21735) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using FLII Polyclonal Antibody (Product # PA5-21735). Sample (50 µg of whole cell lysate). Lane A: Mouse brain . 5% SDS PAGE. FLII Polyclonal Antibody (Product # PA5-21735) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Flightless I in paraformaldehyde-fixed HeLa cells using a Flightless I polyclonal antibody (Product # PA5-21735) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Flightless I in paraformaldehyde-fixed mouse ESC D3 cells using a Flightless I polyclonal antibody (Product # PA5-21735) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC D3, using Flightless I (Product # PA5-21735) antibody at 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Flightless I (Product # PA5-21735) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Product # MA1-25054) (Red) at 1:2,000.
