Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-30032 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FLII Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Raji, mouse brain, rat muscle.
- Concentration
- 0.95 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using FLII Polyclonal Antibody (Product # PA5-30032). Sample (30 µg of whole cell lysate). Lane A: Raji . 5% SDS PAGE. Flightless I antibody . FLII Polyclonal Antibody (Product # PA5-30032) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using FLII Polyclonal Antibody (Product # PA5-30032). Sample (50 µg of whole cell lysate). Lane A: Mouse brain . 5% SDS PAGE. FLII Polyclonal Antibody (Product # PA5-30032) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FLII Polyclonal Antibody detects FLII protein by Western blot analysis. A. 50 µg rat muscle lysate/extract.5 % SDS-PAGE. FLII Polyclonal Antibody (Product # PA5-30032) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FLII Polyclonal Antibody detects FLII protein at cytoplasm by immunofluorescent analysis. Sample: Raji cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FLII protein stained by FLII Polyclonal Antibody (Product # PA5-30032) diluted at 1:500. Blue: Hoechst 33342 staining.