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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- R31062 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- SIP1 Antibody / ZEB2
- Antibody type
- Polyclonal
- Description
- This highly specific SIP1 antibody is suitable for use in Western blot applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the SIP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of SIP1 antibody and Lane 1: 293T; 2: HeLa cell lysate. Predicted molecular weight 120~136 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HEL and 2) MCF7 cell lysate with SIP1 antibody. Predicted molecular weight: 120-136 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE mouse brain tissue with SIP1 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat brain tissue with SIP1 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human glioma tissue with SIP1 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
