H00008721-M03

antibody from Abnova Corporation

Targeting: EDF1 CFAP280, EDF-1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00008721-M03

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00008721-M03, RRID:AB_1674792

Product name

EDF1 monoclonal antibody (M03), clone 3E6

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant EDF1.

Antigen sequence

MAESDWDTVTVLRKKGPTAAQAKSKQAILAAQSRG
EDVETSKKWAAGQNKQHSITKNTAKLDRETEELHH
DRVTLEVGKVIQQGRQSKGLTQKDLATKINEKPQV
IADYESGRAIPNNQVLGKIERAIGLKLRGKDIGKP
IEKGPRAK


Isotype

IgG

Antibody clone number

3E6

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

EDF1 monoclonal antibody (M03), clone 3E6. Western Blot analysis of EDF1 expression in Hela S3 NE(Cat # L013V3 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged EDF1 is 1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to EDF1 on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to EDF1 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol