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- Product number
- PA5-106484 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GRK5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Antibody detects endogenous levels of total GRK5.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Cardiomyocyte Cell-Cycle Regulation in Neonatal Large Mammals: Single Nucleus RNA-Sequencing Data Analysis via an Artificial-Intelligence-Based Pipeline.
Nguyen T, Wei Y, Nakada Y, Zhou Y, Zhang J
Frontiers in bioengineering and biotechnology 2022;10:914450
Frontiers in bioengineering and biotechnology 2022;10:914450
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of GRK5 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with GRK5 polyclonal antibody (Product # PA5-106484) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of GRK5 in human pancreatic cancer. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with GRK5 polyclonal antibody (Product # PA5-106484) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 7 Immunofluorescence analysis confirming GRK5 expression in cardiomyocytes. Here, the expression of GRK5 was represented by the average GRK5 red-channel value (light intensity) that is overlapped with cardiac troponin (cTnT) blue-channel (foreground), which was calculated after adjusting GRK5 red-channel background. DAPI (blue) indicates nuclei. (A) Violinplot of GRK5 intensity in 118 staining images. Here, the horizontal bar and the star (*) indicate nonparametric Wilcoxon's rank sum test between two sample groups. * p -value < 0.05. (B) Representative images of GRK5/cTnT/DAPI in each group.
