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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [3]
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- Product number
- PA5-88213 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CHAMP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LPKSALFSES QKAVELGDEL QIDAIDDQKC DILVQEELLA SPKKLLEDTL FPSSKKLKKD NQESSDAELS SSEYIKTDLD AMDIKGQESS SDQEQVDVES IDFSKENKMD MTSPEQSRNV LQFTEEKEAF ISEEEIAKYM KRGKGKYYCK ICCCRAMKKG AVLHHLVNKH NVHSPYKCTI CGKAFLLESL LKNHVAAHGQ SLLKCPRCNF ESNFPRGFKK HLTHCQSRHN EEANKKLMEA LEPPLEEQQI; Positive Samples: U-87MG, A-431, Jurkat ; Cellular Location: Chromosome, Cytoplasm, Nucleus, centromere, cytoskeleton, kinetochore, spindle
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.55 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using CHAMP1 Polyclonal antibody (Product # PA5-88213) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 1s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CHAMP1 in extracts of various cell lines using CHAMP1 Polyclonal Antibody (Product # PA5-88213) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Hela cells using CHAMP1 Polyclonal antibody (Product # PA5-88213) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of CHAMP1 was performed in Hela cells using CHAMP1 Polyclonal Antibody (Product # PA5-88213) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of CHAMP1 was performed in Hela cells using CHAMP1 Polyclonal Antibody (Product # PA5-88213) at a dilution of 1:100. Blue: DAPI for nuclear staining.
